Among the 19 hub genes, we selected AP3B2, GABRD, GPR158, KIAA0513, and MAL2, which have been little studied on the associations with AD, in order to validate their expressions in cortices of 5xFAD and WT mice and in different Braak stages of AD samples (GSE106241; Marttinen et al., 2019). This evidence concerns the gene GPR158 and Alzheimer disease.