PRDM16 and obesity due to melanocortin 4 receptor deficiency: To explore whether UCP1 was involved in the obesity seen in the DBP exposed offspring, the relative mRNA levels of UCP1, Peroxisome proliferator-activated receptor γ coactivator 1α (Pgc-1α), Transcription factor PR domain containing 16 (Prdm16) and Cell-death inducing DNA fragmentation factor-like effector A (Cidea) in mice were analyzed by RT-PCR, and UCP1 protein expression was evaluated by Western blotting.