BRAF and colorectal carcinoma: Moreover, the highest levels of IL-8 were observed in CRC cell lines carrying both BRAFV600E and PTEN-loss (n = 5): indeed, combined BRAF/PTEN analysis predicted an IL-8 value higher than 46 pg/mL with 87.50% sensitivity and 80.00% specificity (AUC = 0.88) and the ROC curve-based prediction algorithm based on these two alterations had 68% accuracy in predicting IL-8 production (p = 0.002) (Fig. 1b).