To determine the extent of hippocampal neurogenesis in T2DM, we first quantified the number of cells expressing nestin and/or doublecortin (DCX) in the subgranular zone (SGZ) in brain sections of 12-week-old male db/db mice using unbiased stereology (Fig. 1A–D). The gene discussed is DCX; the disease is type 2 diabetes mellitus.