To further verify the antianxiodepressive effect of Sm-VLO projection, we next injected recombinant AAV1-Cre (rAAV-hSyn-Cre) into the bilateral Sm and the AAV-DIO-hM3Dq into the bilateral VLO for specific infection of VLO postsynaptic neurons with hM3Dq or mCherry (Figure 9, G and H). Here, RIC8B is linked to infection.