Moreover, additional immunohistochemistry might be helpful for cases with altered CNVs of EGFR or FGFR. We could detect the somatic driver mutations or germline mutations in five triple-negative cancers; however, the genes covered by the QIAseq Human Breast Cancer Panel might be inadequate for analysis of triple-negative breast cancers because these cancers are known to have highly variable mutations [42]. This evidence concerns the gene EGFR and breast cancer.