In an Rb, p107, and p130 triple-knockout (TKO) HCC model, transcriptional enrichment analysis of HCC cells showed the activation of pathways associated with Notch1-4 signaling and genes that activate canonical oncogenic signals, such as Wnt, p38, Ras, and MAPK [150]. The gene discussed is RB1; the disease is hepatocellular carcinoma.