When comparing SEC + CD44-purified EV microRNA from the serum of glioblastoma patients to HV, we saw significantly higher levels of miR-15b-3p, miR-21-3p, miR-155-5p, and let-7a-5p in glioblastoma patients (p = 0.01, p = 0.001, p = 0.01, and p = 0.008, respectively) and a non-significant trend for miR-23a-3p and miR-106a-5p (p = 0.18 and p = 0.07, respectively), while no significant difference was detected for miR-23a-3p, miR-328-3p, and miR-486-5p (p = 0.45 and p = 0.48 respectively, Figure 3A–H). This evidence concerns the gene CD44 and glioblastoma.