Flow cytometry was performed for T cells (CD4+, CD8+), macrophages (F4/80), and myeloid-derived suppressor cells (MDSC), identified as dual (Gr1+/CD11b+) cells in dissociated DVL3 and TRAMP C1 (gating strategy demonstrated in Supplementary Figure S5), at similar size/ tumor volume for baseline immune profiling (Supplementary Figure S6A). This evidence concerns the gene CD4 and neoplasm.