TP53 and neoplasm: Transgenic tumours from which the DVL3 cells were generated, were derived via deletion of Pten and trp53, genes that are frequently mutated in human PCa and are implicated in aggressive forms of the disease [10]; thus, avoiding the introduction of tumorigenic viral proteins such as SV40 large T-antigen, which was used to generate the TRAMP C1 cell line [6].