FNDC5 and type 2 diabetes mellitus: For instance, Zhu et al. reported that exogenous administration of irisin (0.5 mg/kg body weight, intraperitoneal injection) for 2 weeks improved acetylcholine (ACh)-induced endothelium-dependent relaxation which was diminished by 8 weeks by high fat diet-induced T2D in the mouse aorta ex vivo [9].