IL4 and tuberculosis: To this end, we determined the bacterial content in TB-PE-treated M(IL-4) macrophages, which were exposed (or not) to Orlistat prior to Mtb infection, based on two experimental approaches: i) assessing the area of red fluorescent protein (RFP)-Mtb associated to individual cells by confocal microscopy, and ii) enumerating the CFU recovered from the cultures.