Furthermore, the results from immunostaining showed that PDGFRA-positive cells colocalized with GLI1-positive (LacZ-positive) cells in artery adventitia (Figure 2D, left), and the remarkable induction of PDGFRA and LacZ was found in CKD mice, although the presence of autofluorescence of elastic laminae was also found (Figure 2D, right). This evidence concerns the gene GLI1 and chronic kidney disease.