In accordance with prior genome-wide CRISPR screens and functional studies in B-ALL, many survival dependent genes were identified in the top 50 genes in our screen including PAX5, DOT1L, ZFP64, YY1, MEF2C, MYC, and KMT2A (Gu et al., 2019; Hyle et al., 2019; Pridans et al., 2008; Lu et al., 2018). The gene discussed is YY1; the disease is precursor B-cell acute lymphoblastic leukemia.