Lastly, a transcriptome analysis from the to-date largest human B-ALL transcriptome cohort (N = 1988 patients) (Gu et al., 2019) identified USF2 expression to be significantly correlated with HOXA9 in MLLr-subtype patients (N = 136 patients) (Figure 5—figure supplement 3A-D) highlighting that the USF2 and HOXA9 regulation axis could have clinical relevance for patients in this specific subtype. Here, HOXA9 is linked to acute lymphoblastic leukemia.