In AmphoB-treated Marc-145-IFITM3-flag cells, the DiOC18-PRRSV dequenching rate was quicker than that of untreated cells (Fig. 5F), and the t1/2 for fusion occurred ∼10 min earlier, even though virus trafficking times may vary widely even in synchronized infections (Fig. 5F). This evidence concerns the gene IFITM3 and infection.