In addition, mTORC1 (Figure 3e), TGF-β (Figure 3f), and p53 pathway (Figure 3g) gene sets were also enriched in CTCs and further validation of RNA-seq data using qRT-PCR and a subset of genes from these pathways resulted in a similar trend when compared to HD samples (Figure S5). Here, TGFB1 is linked to Huntington disease.