Through the Hstudio M4 system, Transwell method, three‐dimensional cell culture method, it was found that DSG2 knockdown significantly inhibited the capacities for migration, invasion, and VM in glioma cells compared with the sh‐NC group, and CEBPD overexpression significantly rescued the effect of DSG2 knockdown (online Additional Figure 3A,B and Figure 5G). The gene discussed is CEBPD; the disease is central nervous system cancer.