This study used data from LGG (brain lower grade glioma) and GBM (glioblastoma multiforme) groups in the TCGA database to analyze the genes related to PUM2 (online Additional file 3) and the genes related to UBE2I (online Additional file 4), and to intersect with the transcription factors in the Jaspar database to select the six most relevant transcription factors. The gene discussed is UBE2I; the disease is glioblastoma.