In the measurement of serum concentration of alanine aminotransferase (ALT) there is a statistical difference between SH x IR, which guarantees sufficient ischemia; SH x IRIC, which shows that there is no protection by the drug against induced injury; IR x IRIC shows the worsening effect by Indigo Carmine in increasing the lesion IR (Fig. 3A). The gene discussed is GPT; the disease is ischemia.