Interestingly, TDP-43 was not mislocalized from the nucleus and did not aggregated into cytoplasmic inclusions, indicating that wild-type TDP-43, when overexpressed, is sufficient to induce cellular toxicity and ALS-like phenotypes, but, contrarily to TDP-43 mutants, the wild-type allele retains its nuclear localization and rarely aggregates. The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.