So, the main purpose of this research was to investigate the anticancer properties of Astragalus ovinus extract (AOE) against 7, 12 dimethyl benz (a) anthracene (DMBA)-induced breast cancer in rats, with a particular focus on pathways involved in BC, including the antioxidant-oxidant balance: malondialdehyde (MDA), catalase (CAT), proliferating cell nuclear antigen (PCNA) and extracellular calcium, apoptotic (P53/TP53) and metastatic factors (CA15.3). Here, PCNA is linked to breast cancer.