To evaluate the role of the expression of Gal-3 and Gal-9 during HCC occurrence and their prognostic values, we examined the Gal-3 and Gal-9 protein expression in HCC tumor tissue and paired non-tumor tissue, then explored the association of the protein expression with patient clinical characteristics and prognosis in this study. This evidence concerns the gene LGALS9 and hepatocellular carcinoma.