Profibrogenic genes such as TGFβ, PDGFα (platelets derived growth factor alpha) and connective tissue growth factor (CTGF) which are major indicators of liver fibrosis, were assayed using qPCR and shown to be significantly increased in Mdr2KO mice compared to FVBN controls (Fig. 7E–G). The gene discussed is TGFB1; the disease is Hepatic fibrosis.