In the Long-term insulin (DM) group, the insulin dose required to control glucose levels gradually decreased, and the doses were significantly lower on the 3rd, 4th and 5th days of insulin therapy than on the first day (3rd, 4th, and 5th days vs. first day: p = 0.0001, < 0.0001, and < 0.0001, respectively). This evidence concerns the gene INS and diabetes mellitus.