The univariate analysis showed that age, sex, etiology (HBV), alcohol, underlying liver cirrhosis, tumor number (multiple), tumor size ≥3.2 cm, vascular invasion, extrahepatic metastasis, α-Fetoprotein (AFP) ≥ 100 ng/mL, Protein induced by vitamin K absence or antagonist-II (PIVKA) ≥100 mAU/L, albumin, total bilirubin, prothrombin time, lactate dehydrogenase (LDH), and neutrophil, lymphocyte, monocyte counts were associated with OS in patients with HCC (P < 0.05, Table 2). The gene discussed is AFP; the disease is hepatocellular carcinoma.