In a study from Dang et al., transfected human glioma cell lines with mutant IDH1 were metabolically profiled by LC-MS and showed an accumulation of D2-HG, a product of α-ketoglutarate reduction by the IDH1 mutant, both in cellular extracts and in the culture medium, compared to the wild type cells [133]. This evidence concerns the gene IDH1 and glioma.