In vitro, the expression levels of TLR4, MyD88, NF-κBp65 and NLRP3 protein were detected by Western Blotting in HG-induced glomerular mesangial cells (HBZY-1), and all of them were overexpressed, demonstrating a causative role for TLR4/NF-κB/NLRP3 inflammasome pathway in DKD progression [66]. Here, NFKB1 is linked to diabetic kidney disease.