KEGG enrichment analysis suggests the target proteins of miR-4334-5p were enriched in cGMP-PKG signaling pathway, PI3K-Akt signaling pathway, amoebiasis, chemokine signaling pathway, estrogen signaling pathway, bacterial invasion of epithelial cells, AMPK signaling pathway, Insulin resistance, pathways in cancer, and Apoptosis-multiple species (Supplemental Figure S1B). The gene discussed is AKT1; the disease is amebiasis.