Herein, MG increased ATG5-12, ATG7, Beclin1, ULK1, and LC3-II/I ratio and decreased p62/SQSTM1 and p-mTOR in the liver of Tylo-injected rats and PA-stimulated HepG2 cells, suggesting that MG might promote the formation of autophagic flux to alleviate hepatic steatosis. This evidence concerns the gene ATG5 and fatty liver disease.