CR1 and Alzheimer disease: Although it was low-throughput, we detected decreases in DNA methylation in three out of the six genes, CLU, CR1, and PICALM, whereas no significant DNA methylation changes were observed in APOE, BIN1, and ABCA7. Furthermore, we replicated AD-associated DNA hypomethylation in the CpG island shore of TREM2 in our blood samples using pyrosequencing.