APOE and Alzheimer disease: We selected target regions in the CpG island shores of six of the top seven AD-associated genes in a large scale genome-wide association meta-analysis of clinically diagnosed late-onset AD [31] for methylation analyses, APOE, CLU, CR1, PICALM, BIN1, and ABCA7, as follows: we initially referred to the distribution of CpG dinucleotides along the genes (Fig 1 and S1–S5 Figs).