After the adjustment was made for confounding factors including age, gender, FBG, TC, BMI, TG, ALT, cirrhosis, NAFLD, MetS, HBV infection, alcoholic liver disease, smoking status, drinking status, and physical activity, the association between gallbladder status and PLC was attenuated but remained significant, the corresponding HRs (95%CI) in GSD group and cholecystectomy group were 1.77 (1.05–2.94), 5.25 (1.95–14.17) respectively. The gene discussed is GPT; the disease is Cirrhosis.