Using CRISPR/Cas9 gene editing, we engineered in mouse the same deafness-causing variants as in human TBC1D24. Unexpectedly, Tbc1d24 mutant mice have normal auditory functions even though they have biallelic recessive or dominant missense mutations orthologous to either of two variants associated with human non-syndromic deafness, DFNB86 and DFNA65 (Table 1). This evidence concerns the gene TBC1D24 and deafness.