Other experimental pieces of direct evidence for the participation of FUS in DSB repair come from examination of the effects of a knockdown of FUS by a small interfering RNA or expression of fALS-associated mutant FUS versions (R244C, R514S, H517Q or R521C) in murine primary neuronal culture and/or human osteosarcoma U2OS cells [37]. Here, FUS is linked to osteosarcoma.