In our experiment, we have observed that mice developed with T2DM have demonstrated a constant elevation of fasting blood glucose more than 300 mg/dl throughout the study without fluctuation in plasma insulin level which was 1.26 ± 0.04 ng/mL and 1.34 ± 0.14 ng/mL respectively on day 0 and day 28 of treatment. Here, INS is linked to type 2 diabetes mellitus.