In particular, the constitutive expression of SAMHD1 detected in uninfected HFFs was significantly upregulated upon infection with either AD169 (Fig 2a, 2b and 2d), VR-1814 or TR (Fig 2d and 2e), or fresh primary HCMV isolates collected from infants with congenital infection (Fig 2c). Here, SAMHD1 is linked to infection.