In our study, we show that SAMHD1 mRNA and protein levels significantly increase in response to infection with most of the HCMV strains tested–i.e., AD169, VR-1814 and viral isolates from congenitally infected infants–and in different cell types–i.e., primary fibroblasts, epithelial cells and differentiated THP-1 cells. This evidence concerns the gene SAMHD1 and infection.