Eight of these 59 SNPs showed nominally significant associations with hearing difficulty in the UK Biobank (p < 0.05; S8 Table), including both loci that reached genome-wide significance in the largest previous GWAS of ARHI[4]: rs4932196, 54 kb 3' of ISG20 (p = 2.6x10-5 in the UK Biobank); and rs5750477, in an intron of TRIOBP (p = 1.3x10-6). Here, ISG20 is linked to presbycusis.