To identify the proteome-wide changes in ubiquitylated proteins associated with the oncogenic tumor function of FBXO2, we isolated the FBXO2 protein complex from Ishikawa-ovFBXO2 and Ishikawa-NC stable cell lines mixed with anti-ubiquitin remnant motif, and determined the proteins present in the complex by using liquid chromatograph-tandem mass spectrometry (LC MS/MS) to interrogate the differences in the proteome and ubiquitylome between Ishikawa-NC and Ishikawa-ovFBXO2 cells. The gene discussed is FBXO2; the disease is neoplasm.