The feature plots and spatial feature plots were utilized to illustrate the distribution and expression of CIRBP, FAM110B, ACAA1, ACAT1, and DHCR7, showing that these key genes were highly expressed in the invasive ductal carcinoma tissue (cluster 2, 5, 8) (Figures 9B,C). This evidence concerns the gene ACAA1 and invasive ductal breast carcinoma.