Taken together, our work clearly identified a complex genome-wide IGF2BP3-RNA interaction map in human lung cancer cell line and indicated that IGF2BP3 binds to CDS regions and regulates multiple alternative splicing events, which will help to understand the regulatory mechanisms of IGF2BP3 at the pre-RNA splicing level and provide insight into the role of IGF2BP3 in multiple biological processes. Here, IGF2BP3 is linked to lung cancer.