To further prove that SRD5A1 is the carcinogenic driver for CRC, SRD5A1 endogenous expression was functionally knocked down using shSRD5A1 plasmid transfection technology in the HCT116 and LOVO CRC cell lines, which was validated by Western blot (Figure 2A). Here, SRD5A1 is linked to colorectal carcinoma.