SRD5A1 and colorectal carcinoma: After being cultured for 48 and 72 h, the MTT assay demonstrated that SRD5A1-silenced CRC cells exhibited significantly impaired cell viability in both HCT116 and LOVO cells compared with the control (P < 0.01; Figure 2B), indicating that SRD5A1 is required for the maintenance of CRC cell viability.