In this study, we found that the expression of FGF-2, PDGFB related to tumor angiogenesis was significantly decreased after knocking out EGFL6 in vivo and vitro. Furthermore, the results of heat map analysis showed that the expression of FGF-2, FGFR-2, PDGFB, PDGFR, and other growth factors were downregulated after the knockout of EGFL6. This evidence concerns the gene PDGFB and neoplasm.