In this study, the EGFL6 was knocked out by CRISPR/Cas9 in an ovarian cancer cell line, using a specific guide RNA (gRNA) designed to target the exons of EGFL6. Further, the role of EGFL6 in the proliferation and migration of cells, tumor growth, and angiogenesis in ovarian cancer was assayed. This evidence concerns the gene EGFL6 and ovarian cancer.