PDCD1 and melanoma: Next, we overlapped our DEGs of tumors treated with SAM+anti-PD-1 (compared to control) with known melanoma cancer genes from The Melanoma Gene Database (MGDB) that has 422 melanoma-specific protein-coding genes (41) and The Cancer Genome Atlas (TCGA) (Figure 3D and Supplementary Figure 3) (42, 43).