Vaginal epithelial cells lines (VECs), primary amnion epithelial cells (AECs) and primary myocytes (ascending infection model), or peripheral blood mononuclear cells and placental explants (haematogenous model) were primed with the TLR3 agonist poly I:C prior to treatment with the TLR2 agonist heat-killed Listeria Monocytogenes (HKLM) or TLR2/6 agonist FSL-1 to determine their effect on pro-inflammatory and pro-labour mediators. This evidence concerns the gene TLR3 and infection.