LAMP1 and melanoma: For this purpose, we cultured Vγ9Vδ2 T cells for 5 h in the presence of Brefeldin A and anti-CD107a antibody in different conditions: (i) with cell culture medium (as a negative/baseline control), (ii) with FM-28 melanoma cells, (iii) with FM-28/ZOL melanoma cells, or (iv) with PMA/ionomycin (as a positive control).