TGFB1 and neoplasm: Given our previous observations that human tumor antigen-specific regulatory populations can utilize IL-35, and that they are distinct from those utilizing TGF-β or IL-10 (48), then if it can be shown that such human Tregs also produce EV associated IL-35, similar to mice (19), it suggests that these IL-35+ Tregs may be able to propagate infectious tolerance via a previously uninvestigated mechanism such as IL-35+ EVs.