One study used an optimized cationic lipid-assisted nanoparticle (CLAN) system that can encapsulate mCas9 and guide NLRP3 (CLANmCas9/gNLRP3), to disrupt NLRP3 gene expression in bone marrow derived macrophages (BMDM) in vitro, and in vivo when administered by injections in murine models of LPS-induced septic shock, MSU-induced peritonitis and high-fat-diet-induced diabetes (52). The gene discussed is NLRP3; the disease is septic shock.