Firstly, we used hematoxylin and eosin (HE) staining and immunohistochemical staining of CD68 to detect the total macrophages of the aortic wall as well as specific macrophage markers (CD45+F4/80+CD11b+) by FCM to detect the splenic macrophages in the experimental AAA model. Here, PTPRC is linked to triple-A syndrome.