By comparing the immune and stroma contents in immune-active and tolerant tumors with immune-inactive tumors and normal tissues, macrophages, endothelial cells and cancer associated fibroblasts (CAFs) were observed as significantly elevated cell types in the former group (Additional file 9: Figure S5b) whereas CD4 positive T cells are depleted, indicating macrophages and inflammation plays important role in these tumors. Here, CD4 is linked to cancer.