Finally, CD8.2 displayed a quiescent, central-memory CD8+ T-cell phenotype with expression of LEF1, SELL, IL7R, and LTB. In contrast to Fernandez et al7 and previous scRNA-seq data obtained from various cancers, we did not detect a clear exhausted phenotype in the CD8+ T cells.35–37 The CD8 clusters with reduced cytotoxic potential show expression of CD69, suggesting recent T cell receptor (TCR) activation and it will be of future interest to examine how these CD8+ populations were activated and how they affect the pathogenesis of atherosclerosis. The gene discussed is LEF1; the disease is cancer.