Considering EDN insult caused activation of JUN in 30% of RGCs (Fig. 2a–c) and 69% of cCASP3+ RGCs were also JUN+ after EDN1 insult (Fig. 2d–f), it remained possible that JUN regulates EDN1-induced RGC death, similar to glaucoma-relevant RGC death. This evidence concerns the gene EDN1 and glaucoma.