Furthermore, DNA replication, regulation of cell cycle G2M phase transition, bladder cancer, colorectal cancer, non‐small cell lung cancer, renal cell carcinoma, MTOR signaling pathway, p53 signaling pathway, and WNT signaling pathway were the main GO and KEGG terms enriched in the high POU5F1 phenotype according to the GSEA. The gene discussed is TP53; the disease is hereditary clear cell renal cell carcinoma.