NFE2L2 and melanoma: NFE2L2 knockdown furthermore resulted in an enhanced sensitivity toward oxidative stress (Fig. 2b), elevated ROS levels (Fig. 2c) and reduced intracellular glutathione content (Fig. 2d), as determined in UACC-62 cells, confirming that NRF2 is required for the maintenance of the intracellular redox balance in melanoma cells.